SOX4 Antibody Used for Western Blot Analysis of Glioma Cell Lysate

August 11, 2020

Centre for Cellular and Molecular Medicine
Ludwig Cancer Research
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

SOX4 Antibody

Catalog Number:

NBP1-50776

Clone Number:

N/A

Our aim was to check the levels of SOX4 in glioma cell lysates. Briefly, cells were lysed in ripa buffer, centrifuged and supernatant was collected. Loaded 30ug protein in SDS-PAGE and analyzed the protein levels by Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Glioma cells

Primary Incubation

1:1000 in 5% BSA, overnight in cold room

Blocking Agent

5% BSA

Secondary Incubation

1:10000 in 5%BSA

Tertiary Incubation

N/A

Detection

Chemi doc, femto reagent

Results Summary

Observed a clear specific band corresponding to SOX4 at approx 50KD.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

good antibody for western blot

The Bad

N/A

The Bottom Line

Good antibody and easy to standardise for western blot

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