Western Blot Analysis of Glioma Cell Lysate with GAPDH Antibody

August 12, 2020

Centre for Cellular and Molecular Medicine
Ludwig Cancer Research
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

SQSTM1/p62 Antibody

Catalog Number:

5114S

Clone Number:

6

Our aim is to check the levels of P62 protein in Cell Lysates transfected with shRNA. Briefly, cells were either untransfected or transfected with shRNA against P62 and endogenous P62 protein levels was analyzed with Western Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Glioma cells

Primary Incubation

1:1000 in 5% BSA, overnight in cold room

Blocking Agent

5% BSA

Secondary Incubation

1:10000 in 5%BSA

Tertiary Incubation

N/A

Detection

Chemi doc, femto reagent

Results Summary

A specific band corresponsind to P62 was observed by Western Blot and the levels were reduced in shRNA transfected cells.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Good antibody for Western Blot.

The Bad

N/A

The Bottom Line

Excellent antibody and easy to optimize for Western Blot.

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