Cell Signaling Technology
SQSTM1/p62 Antibody
5114S
6
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Our aim is to check the levels of P62 protein in Cell Lysates transfected with shRNA. Briefly, cells were either untransfected or transfected with shRNA against P62 and endogenous P62 protein levels was analyzed with Western Blot.
Western Blot
Glioma cells
1:1000 in 5% BSA, overnight in cold room
5% BSA
1:10000 in 5%BSA
N/A
Chemi doc, femto reagent
A specific band corresponsind to P62 was observed by Western Blot and the levels were reduced in shRNA transfected cells.
Good antibody for Western Blot.
Excellent antibody and easy to optimize for Western Blot.