SKP2 Antibody for Western Blot Analysis of Glioma Stem Cell Lysate

August 12, 2020

Centre for Cellular and Molecular Medicine
Ludwig Cancer Research
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

R&D systems

Product Name:

Human Skp2 Antibody

Catalog Number:

MAB6817

Clone Number:

CFTD012003A

Our aim is to check the levels of SKP2 protein in glioma stem cell lysates. Briefly, cells were lysed in RIPA buffer, centrifuged and supernatant was collected. Loaded 30 ug of protein on SDS-PAGE.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Glioma stem cell whole cell lysates

Primary Incubation

1:1000 in 5% BSA, overnight in cold room

Blocking Agent

5% BSA

Secondary Incubation

1:10000 in 5%BSA

Tertiary Incubation

N/A

Detection

Chemi doc, femto reagent

Results Summary

A single specific band corresponding to SKP2 was observed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody for Western Blot

The Bad

N/A

The Bottom Line

Good antibody for Western Blot analysis.

Share your experience with other scientists. Write a Review! »

Join the discussion