Rat anti-MBP Antibody for Immunofluorescence

August 25, 2020

Angioscience
ECAS
Graduate Student

Overall

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Company:

AbD Serotec

Product Name:

Rat anti-MBP antibody

Catalog Number:

MCA409S

Clone Number:

N/A

Antibody labels specifically MBP with low amount of background straining. Recommended.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Adult mouse spinal cord.

Primary Incubation

1:100, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

The antibody correctly labels MBP surrounding the neurons. Low background staining is observed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Quality.

The Bad

None.

The Bottom Line

Recommended antibody for studying MBP.

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