Rabbit anti-Caspase-3 cleaved (Asp175) Antibody for Immunofluorescence

August 13, 2020

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Rabbit anti-Caspase-3 cleaved (Asp175) antibody

Catalog Number:

9661

Clone Number:

N/A

This antibody works really well to detect cells in apoptosis during development. Easy to optimize.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryo E11.5: Spinal cord

Primary Incubation

1:200, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

1:500, 2h at RT, 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

Not done.

Detection

Confocal microscope.

Results Summary

This antibody detects cells which are entering in apoptosis. In the developing spinal cord, however, some non-dying cells express it too. The overall staining works super well and easy to optimize and adapt the protocol.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

A little bit of background.

The Bottom Line

This product is recommended for researchers studying development.

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