Pretty Good Anti-human IgD

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human IgD

Catalog Number:

555779

Clone Number:

IA6-2

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining IgD in B cell (CD19+) of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs from a healthy donor

Primary Incubation

anti-IgD PE 1/50, 20', 4°C

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain IgD in B cells, as can be observed in the attached figure.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works well.

The Bad

The price!

The Bottom Line

It works.

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