Good Anti-human CD20

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biosciences

Product Name:

FITC Mouse Anti-Human CD20

Catalog Number:

555622

Clone Number:

2H7

Info:Supplier Page 

Browse Similar Products: CD20 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+CD20+) in isolated PBMCs from peripheral blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

Anti-CD20 1/50, 20', 4°C

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain B cells (CD20+) in PBMCs of a healthy donor (HD).

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works nicely.

The Bad

It is not very bright.

The Bottom Line

Can be used!

Share your experience with other scientists. Write a Review! »

Join the discussion