Excellent Antibody for Analyzing Blood Leukocytes

August 03, 2020

Pediatrics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

BV605 anti-human CD45

Catalog Number:

304042

Clone Number:

HI30

anti-CD45 antibody was used to stain blood for identification of major leukocyte populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Venous Blood

Primary Incubation

2.5 uL of antibody added to 50 uL of blood

Blocking Agent

Fc receptor blocking before staining

Secondary Incubation

None

Tertiary Incubation

None

Detection

V 610/20 filter on BD FACSYMPHONY

Results Summary

Human venous blood was obtained and stained for analysis by flow cytometry. After exclusion of debris, sample was plotted as side scatter over CD45 for gating on the 4 major leukocyte populations in blood. Each population was then selected for further analysis

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable fluorescence, not lost upon fixation. The stain is bright

The Bad

The brilliant violet conjugates are more expensive than other fluorochromes. Some spillover into other violet channels

The Bottom Line

Great antibody for analysis of multiple blood leukocyte populations.

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