Good Antibody for Staining Human HSPCs from Peripheral Blood

July 27, 2020

Experimental Hematology
Department of Biomedicine, University Hospital Basel
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 605™ anti-human CD123 Antibody

Catalog Number:

306026

Clone Number:

6H6

We analyzed the expression of CD123 in HSPCs from Human PBMC samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

5 µl per million cells in 100 µl staining volume, RT 30 minutes

Blocking Agent

Human Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry, BD Fortessa

Results Summary

I used this antibody to FACS stain PBMC sample from patients. I used the concentration specified by the company and stained the cells at RT for 30 minutes post Fc Block.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good antibody for human HSPCs

The Bad

Nothing

The Bottom Line

Recommended!

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