Good Antibody from R&D for Staining Human HSCs

July 29, 2020

Experimental Hematology
Department of Biomedicine, University Hospital Basel
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D

Product Name:

Human IL-1 RI PE-conjugated Antibody

Catalog Number:

FAB269P-100

Clone Number:

N/A

I used this antibody to FACS stain PBMC sample from patients. I used the concentration specified by the company and stained the cells at RT for 30 minutes post Fc Block.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

10ul/million cells, 30 minutes, RT

Blocking Agent

Human Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Expression of IL1R1 was analyzed on peripheral blood HSCs and HSPCs from patient PBMC samples.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works perfectly for Human HSCs

The Bad

It's a little expensive.

The Bottom Line

Recommended.

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