IRDye 680RD Secondary Antibody Works Well for Immunoblotting

July 13, 2020

Department of Cell Biology
Johns Hopkins University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

LI-COR Biosciences

Product Name:

IRDye 680RD Goat anti-Rat Secondary Antibody

Catalog Number:

925-68076

Clone Number:

Our lab used the IRDye 680RD secondary antibody for Western Blotting in the 700nm channel of Odyssey® Imaging Systems. These antibodies can be used for two-color detection when multiplexed with IRDye 800CW secondary antibodies. The signal is strong.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines and Dictyostelium cells

Primary Incubation

1:1000 4 degree over night

Blocking Agent

5% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

N/A

Detection

LI-COR Odyssey CLx

Results Summary

The results are great. The signal is strong and antibodies can be used for two-color detection when multiplexed with IRDye 800CW secondary antibodies.

DOI or PMID #

30194235

Image Gallery

Summary

The Good

Reliable, high quality, user friendly

The Bad

none

The Bottom Line

I highly recommend this secondary antibody for Immunoblotting. It works very well and is user friendly.

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