Miltenyi Biotec
CD19 MicroBeads, mouse
130-121-301
Info: Supplier Page
A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD19+ B cells were isolated from this single-cell suspension using the CD19 MicroBeads, and autoMACS® Pro Separator. Before CD19 microbead based isolation, there were two populations of cells with CD45 population. After positive isolation for CD19, single homogeneous populations of CD19 B-cells were obtained.
Enrichment or isolation of CD19 mouse B-cells
Mouse spleen cell suspensions
None
Using CD19 micro beads with the gentleMACS™ Dissociator and autoMACS® Pro Separator, single homogeneous population of CD19 B-cells were positively enriched.
Effective and specific for CD19 enrichment
Overall, CD19 micro beads gave me sufficient numbers of CD19 B-cells from mouse spleen single cell suspensions.