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Brilliant Violet 650 anti-human CD63
353026
H5C6
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I used this antibody to stain for CD63, a marker of primary granule exocytosis, on neutrophils from blood and sputum of an adult CF patient
Flow Cytometry
Blood, sputum
2.5 uL of antibody added to 2x10^5 cells in 50 uL of PBS+EDTA
Fc receptor blocking before antibody stain
none
Flow cytometry; V670/30 filter on BD Symphony
I used this antibody to compare degranulation by blood and airway neutrophils. Blood neutrophils are mostly negative for CD63, indicating minimal exocytosis of primary granules. Airway neutrophils from sputum demonstrate 3-4 log increase in expression of CD63, indicating significant primary granule exocytosis.
Reliable staining and minimal loss of fluorescence following fixation
Recommend for analysis of human neutrophil degranulation