Anti-Mouse Secondary Antibody Works Well in 800nm Channel

July 14, 2020

Department of Cell Biology
Johns Hopkins University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

LI-COR Biosciences

Product Name:

IRDye 800CW Goat anti-Mouse IgG Secondary Antibody

Catalog Number:

925-32210

Our lab uses this IRDye 800CW Goat anti-Mouse IgG Secondary Antibody for immunoblotting in the 800nm channel of Odyssey® Imaging Systems. We use this for two-color detection when multiplexed with IRDye 680RD secondary antibodies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines and Dictyostelium cells

Primary Incubation

1:1000 4 degree over night

Blocking Agent

5% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

N/A

Detection

LI-COR Odyssey CLx

Results Summary

Results are great. I always get low background and high sensitivity in the 800nm channel.

DOI or PMID #

30194235

Image Gallery

Summary

The Good

Reliable, high sensitivity

The Bad

none

The Bottom Line

I highly recommend this for Immunoblotting. It works very well. This can be used for two-color detection when multiplexed with IRDye 680RD secondary antibodies

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