Great Antibody for Examining Activation

July 02, 2020

Microbiology and Immunology
Columbia University Irving Medical Center
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PerCP/Cyanine5.5 CD95 (Fas) Antibody

Catalog Number:

152610

Clone Number:

SA367H8

Our lab studies T cell biology and we are interested in TCR induced activation of T cells in both mouse and human. We examined the expression of CD95 upon in vitro stimulation of mouse T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse, splenocytes and lymph node cells

Primary Incubation

1:200, 30 min on ice

Blocking Agent

none

Secondary Incubation

none

Tertiary Incubation

none

Detection

BD LSRII

Results Summary

Useful antibody to examine activation of T cells. T cells expressed high levels of CD95 upon stimulation (red) overnight.

Image Gallery

Summary

The Good

Worked well and is bright

The Bad

Has some background

The Bottom Line

Good CD95 antibody that can be used to examine activation of mouse T cells

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