Great IRF4 Antibody for Studying T Cell Activation

July 02, 2020

Microbiology and Immunology
Columbia University Irving Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

IRF4 Monoclonal Antibody (3E4), FITC, eBioscience™

Catalog Number:

11-9858-82

Clone Number:

3E4

Our lab studies T cell biology and we are interested in TCR induced activation of T cells in both mouse and human. We examined the expression of IRF4 upon in vitro stimulation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse, splenocytes and lymph node cells

Primary Incubation

1:200, 30 min on ice after fixation/permeabilization with Tonbo's Foxp3 Fix:perm buffer

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

We saw increased IRF4 expression upon stimulation (red) compared to unstimulated T cells.

Image Gallery

Summary

The Good

Worked well

The Bad

Has high background, needs to be titered accordingly

The Bottom Line

Useful antibody to examine activation of T cells.

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