Measuring TCR Induced Activation in Mouse Cells

Microbiology and Immunology
Columbia
Postdoctoral Researcher

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Company:

Invitrogen

Product Name:

Nur77

Catalog Number:

46-5965-82

Clone Number:

12.14

Our lab studies T cell biology in both human and mouse. We are interested in TCR induced activation of T cells. We examined the expression of "Nur77" upon in vitro stimulation of OTII T cells with its peptide.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse, splenocytes and lymph node cells

Primary Incubation

1:200, 30 min on ice after fixation/permeabilization with Tonbo's Foxp3 Fix:perm buffer

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSRII

Results Summary

There was expected increased expression of Nur77 upon stimulation of OTII T cells with cognate antigen.

Image Gallery

Summary

The Good

Worked well

The Bad

na

The Bottom Line

Works well to examine TCR induced activation in mouse cells.

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