Good Yield for High-throughput Sequencing

July 23, 2020

Tufts University School of Medicine
Molecular Microbiology
Graduate Student

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Company:

Qiagen

Product Name:

DNeasy Blood & Tissue Kit

Catalog Number:

69504

We regularly use this kit to isolate genomic DNA of bacterial transposon libraries. Because there are many unique mutants throughout the library, we need a high genomic DNA yield of each sample to have good representation of the mutant ratios present in the library.

Experimental Design and Results Summary

Application

Genomic DNA Isolation of Bacteria

Starting Material

Bacterial pellet

Tips

Include a bead beating step for Gram positive bacteria

Results Summary

This kit works well because it gives a high yield which allows us to isolate gDNA from a large number of bacteria within the library.Genomic DNA collected from this kit shows minimal denaturation, leaving the majority of the gDNA as a large product at the top of the gel.

DOI or PMID #

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Summary

The Good

High yield and straightforward steps

The Bad

Many consumables and we often run out of the buffer AL before columns have been used up.

The Bottom Line

This is a useful kit for genomic DNA isolation of high complexity samples for high throughput sequencing. It works for both Gram positive and Gram negative bacteria and provides a high yield for both, provided that you include a bead beating step for the Gram positive.

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