Anti-Chk2 Antibody that Works Great for Immunoblotting

June 26, 2020

Department of Cell Biology
Johns Hopkins University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Chk2 Antibody

Catalog Number:

2662

I used this antibody to detect Chk2 levels and DNA damage in various cell lines using Immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:500 4 degrees overnight

Blocking Agent

5% BSA

Secondary Incubation

1:10000 RT 1h

Tertiary Incubation

N/A

Detection

LI-COR Odyssey CLx

Results Summary

The band of this antibody is not strong. There is one band at expected molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific band for target protein

The Bad

The band is not very strong

The Bottom Line

I recommend this antibody for Immunoblotting but the band is not strong.

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