Miltenyi Biotec
B Cell Isolation Kit, mouse
130-090-862
Info: Supplier Page
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at purifying untouched B cells form mice spleens to in vitro culture.
B cell purification
C57B/L6 spleens
Follow the provided protocol. The indicated times are enough.
We could purify untouched B cells with very good purity. There are some B cells that are lost and stay in the column.
The purity achieved is very good and the B cells are untouched.
Lost of B cells in the purification column
Very good option to purified mouse B cells.