Protein Deglycosylation Mix II (P6044S) Effectively Removes Sugars from Antibodies

AbbVie
Biologics
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England BioLabs

Product Name:

Protein Deglycosylation Mix II

Catalog Number:

P6044S

The kit is useful at removing even more stubborn sugars from antibodies for easier mass spec confirmation

Experimental Design and Results Summary

Application

Deglycosylation for Mass Confirmation of Antibodies

Starting Material

Antibodies

Tips

Vortex degly mix buffer II before use

Results Summary

All sugars are removed.

DOI or PMID #

N/A

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Summary

The Good

It is a simple kit with straight forward instructions.

The Bad

Sometimes requires long incubation period for full sugar removal.

The Bottom Line

The kit is effective at removing O-linked and N-linked glycosylations.

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