Specific and Distinct FoxP3 Expression

June 04, 2020

Oncology
Johns Hopkins School of Medicine
Lab Technician

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse FOXP3 Antibody

Catalog Number:

126419

Clone Number:

MF-14

There has been increasing interest in looking at FoxP3 expression in Tregs upon rapamycin treatment, therefore our lab used this antibody to see the expression differences.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:1000

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow

Results Summary

Our results show that upon activation in presence of rapamycin, there is a shift in expression of FoxP3 in our CD4+s T cells that are pushed to Tregs. This antibody works well to see the spread of expression differences.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Distinct expression

The Bad

None

The Bottom Line

If you are interested in looking at the expression of FoxP3, this antibody will work well.

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