Cell Signaling Technology
Akt (pan) (C67E7) Rabbit mAb
4691
C67E7
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I use this antibody to detect total AKT protein level in the cell by western blot which serves as an internal control for AKT phosphorylation events in the pathway
Western Blot
Human cell lines
1:1000 4 ℃ overnight
5% milk
1:2000 room temp 1 hour
None
ECL
The western blot results showed strong and specific bands at expected molecular weight.
N/A
Strong and specific signal
I would recommend this AKT antibody for western blot analysis. Since AKT is heavily modified, it is critical to choose the correct antibody to get accurate quantification of the signaling events.