Good Antibody for PTEN in Western Blot Experiments

June 23, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

PTEN antibody

Catalog Number:

sc-7974

Clone Number:

A2B1

I use this antibody to detect endogenous PTEN from various cell lines. It works well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines

Primary Incubation

1:100 4 ℃ overnight

Blocking Agent

5% milk

Secondary Incubation

1:2000 room temp 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

I got pecific bands at the expected molecular weight. Depending on the cell line used, the signal could be strong or weak.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific signal

The Bad

Not very strong

The Bottom Line

I would recommend this antibody for western blot detection of PTEN in the cell.

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