PAX6 Antibody for Flow Cytometry as a Neural Progenitor Cell Marker

June 04, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

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Company:

BD Biosciences

Product Name:

Alexa Fluor® 647 Mouse Anti-Human Pax-6 Clone O18-1330 (RUO)

Catalog Number:

562249

Clone Number:

O18-1330

Browse Similar Products: Pax-6 Flow Cytometry Antibodies

I use this antibody to test the differentiation efficiency of embryonic stem cells to neural projenitor cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

H9 cell line

Primary Incubation

1:25, room temperature, 45 minutes

Blocking Agent

FACS buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

When embryonic stem cells were induced to differentiate to neural projenitor cells, they became PAX6 positive as indicated by the antibody staining flow cytometry experiment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommend this antibody for PAX6 staining if you are doing NPC differentiation or other experiments involving PAX6 as a marker.

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