Best HRP to Detect Biotinylated Antibodies

May 20, 2020

Pharmaceutical and Biomedical Sciences
College of Pharmacy
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

HRP Streptavidin

Catalog Number:

405210

Streptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, IHC or Western blotting.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse brain

Primary Incubation

1:200

Blocking Agent

Blocking Buffer (BSA, Donkey Serum and Triton X100)

Secondary Incubation

1hr

Tertiary Incubation

5 min

Detection

Immunofluorescence

Results Summary

Dark staining of the targeted antigen

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works really well

The Bad

Nothing

The Bottom Line

Recommended.

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