Brilliant Violet 785™ Anti-Mouse I-A/I-E Antibody

May 27, 2020

Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-mouse I-A/I-E Antibody

Catalog Number:

107645

Clone Number:

M5/114.15.2

This antibody was used to identify the MHC-II+ cells in the brain of BL6 mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine brain tissue

Primary Incubation

20mins 37oC

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS BD LSRFortessa, software FACSDiva

Results Summary

The antibody successfully recognized the cellular populations expressing MHC class II molecules, with high specificity and resolution.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would recommend it.

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