Great Antibody to Detect CD44 Expression on Cells via Flow Cytometry

June 03, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

BV711 Rat Anti-Mouse CD44

Catalog Number:

563971

Clone Number:

IM7

Browse Similar Products: CD44 Flow Cytometry Antibodies

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify antigen experienced and activated T cells in flow cytometry experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500, 1000)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

Splenic cells are strongly and specifically labelled with CD44 to identify populations with low and high expression to mark antigen experienced cells.

DOI or PMID #

N/A

Additional Notes

Accordingly Titrate Antibody based on flow analyzer.

Related Categories

Image Gallery

Summary

The Good

Reliable results as easy to idenitify CD44 expression on splenic cells.

The Bad

None

The Bottom Line

I would recommend this antibody to anyone needed to analyze CD44 expression on T cells. It gives a reliable and strong labeling of CD44 on cells for flow cytometry analysis. You can detect a nice degree of expression from low to high.

Share your experience with other scientists. Write a Review! »

Join the discussion