Good Antibody to Detect CD39 Expression on Cells via Flow Cytometry

June 01, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

CD39 Monoclonal Antibody (24DMS1), PE-Cyanine7

Catalog Number:

25-0391-82

Clone Number:

24DMS1

We are an immunology lab that looks at immune responses in the context of inflammation and cancer. This marker is used to widely used to identify CD39 expression on T cells and other non-immune cells in flow cytometry experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes (Naive and Infected)

Primary Incubation

Multiple Dilutions (1:250, 500)

Blocking Agent

FC Block 100ng/mL in FACS solution (surface)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Cytek Aurora (BVR)

Results Summary

Splenic cells strongly and specifically label with CD39. You can detect different populations based on CD39 and side scatter profile.

DOI or PMID #

N/A

Additional Notes

Accordingly Titrate Antibody based on flow analyzer.

Related Categories

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Summary

The Good

Reliable results as easy to idenitify CD39 expression on splenic cells.

The Bad

None

The Bottom Line

I would recommend this antibody to anyone needed to assess CD39 expression on cells. It gives a reliable and strong labeling of CD39 on cells for flow cytometry analysis.

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