Great Antibody for Perforin from BioLegend

June 03, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse Perforin Antibody

Catalog Number:

154306

Clone Number:

S16009A

We are trying to study CD8 T cell differentiation upon viral infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

Fc block

Blocking Agent

FACS buffer

Secondary Incubation

Surface staining

Tertiary Incubation

ICS at RT for 1hr

Detection

Flow cytometry

Results Summary

We are able to detect perforin positive CD8 T cells upon ex vivo restim.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very bright staining

The Bad

None

The Bottom Line

Overall great antibody to stain for perforin.

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