Decent Antibody for CD27 from Thermo Fisher Scientific

May 28, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD27 Monoclonal Antibody (LG.7F9), FITC, eBioscience™

Catalog Number:

11-0271-82

Clone Number:

LG.7F9

Browse Similar Products: CD27 Flow Cytometry Antibodies

We are trying to study CD8 T cell differentiation in response to viral infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

Fc block

Blocking Agent

FACS buffer

Secondary Incubation

Surface staining at 4C 20min

Tertiary Incubation

Fix

Detection

Flow cytometry

Results Summary

We are able to detect CD27 positive cell at memory stage.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Able to distinguish population

The Bad

None

The Bottom Line

A little bit dim in my opinion. Still not a bad option for the available fluorochrome.

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