Very Good Antibody for Thy-1.1 from BioLegend

May 26, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Catalog Number:

202528

Clone Number:

OX-7

We are trying to stain for adoptively transferred CD8 T cells in response to infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen

Primary Incubation

Fc block for 15min at 4C

Blocking Agent

FACS buffer

Secondary Incubation

Surface staining at 4C for 20min

Tertiary Incubation

Fix

Detection

Flow cytometry

Results Summary

The antibody is amazing. Need to carefully titrate to avoid spilling.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good good antibody and specific.

The Bad

None

The Bottom Line

Very useful antibody on a dim channel.

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