Great Antibody for Phospho-Akt Ser473

May 22, 2020

Immunology
University of Pittsburgh
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

Catalog Number:

4060

Clone Number:

D9E

We use this antibody to detect activation of T-cells via Akt phosphorylation at S473. This antibody is very effective at detecting and reporting these findings.

Experimental Design and Results Summary

Applications

Western Blot

Sample

D.10 and LK35.2 coculture

Primary Incubation

1:1000 in TBS-t overnight at 4C

Blocking Agent

5% BSA for 1h at room temp

Secondary Incubation

1:3000 anti-rabbit HRP-linked 1h at room temp

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

LK35.2 pulsed overnight with doses of conalbumin were incubated with D.10 cells. The lysates showed dose dependent increase of phospho-Akt S473.

DOI or PMID #

10.1074/jbc.RA118.006524

Additional Notes

This clone has other conjugates that we have also found to work quite well in varying applications.

Image Gallery

Summary

The Good

Great and consistent detection of pAkt-S473

The Bad

None

The Bottom Line

A reliable antibody for easy detection of phospho-Akt S473.

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