Cell Signaling Technology
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
4060
D9E
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We use this antibody to detect activation of T-cells via Akt phosphorylation at S473. This antibody is very effective at detecting and reporting these findings.
Western Blot
D.10 and LK35.2 coculture
1:1000 in TBS-t overnight at 4C
5% BSA for 1h at room temp
1:3000 anti-rabbit HRP-linked 1h at room temp
N/A
Chemiluminescence
LK35.2 pulsed overnight with doses of conalbumin were incubated with D.10 cells. The lysates showed dose dependent increase of phospho-Akt S473.
10.1074/jbc.RA118.006524
This clone has other conjugates that we have also found to work quite well in varying applications.
Great and consistent detection of pAkt-S473
None
A reliable antibody for easy detection of phospho-Akt S473.