This BioLegend Antibody Stains Ly6C+ Cells Very Well

June 11, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse Ly-6C Antibody

Catalog Number:

128012

Clone Number:

HK1.4

We wanted to study myeloid cells in the disease vs healthy state, so we used the antibody to stain Ly6C+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains Ly6C+ cells very well and distinguishes the different levels of expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains Ly6C+ cells very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identifying Ly6C+ myeloid cells.

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