This Thermo Fisher Antibody Is Great for Identifying Pro-IL-1b+ Cells

June 05, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), APC, eBioscience™

Catalog Number:

17-7114-80

Clone Number:

NJTEN3

Browse Similar Products: Pro-IL-1b Flow Cytometry Antibodies

We wanted to study IL-1b cytokine secretion in the disease vs healthy state, so we used this antibody to identify pro-IL-1b+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody is great for identifying pro-IL-1b+ cells clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies pro-IL-1b_+ cells well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying cells capable of IL-1b secretion.

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