This BioLegend Antibody Is Great for Identifying CD25+ T Cells

June 03, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse CD25 Antibody

Catalog Number:

102016

Clone Number:

PC61

We wanted to study T cell activation in the disease vs healthy state, so we used the antibody to stain for CD25+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains CD25+ T cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies CD25+ T cells very clearly

The Bad

None to mentione

The Bottom Line

The antibody is great for identifying activated T cells.

Share your experience with other scientists. Write a Review! »

Join the discussion