This BioLegend Antibody Stains Gzmb+ T Cells Very Well

June 01, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human/mouse Granzyme B Recombinant Antibody

Catalog Number:

372208

Clone Number:

QA16A02

We wanted to study cytotoxic T cells in the healthy vs disease state, so we used the antibody to stain for Gzmb+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies Gzmb+ T cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody stains Gzmb+ T cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying Gzmb+ populations of cells in the tissue.

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