This BioLegend Antibody Identifies MHC II+ Cells Very Well

May 14, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse I-A/I-E Antibody

Catalog Number:

107622

Clone Number:

M5/114.15.2

We wanted to study how immune cells changed in the disease vs healthy state, so we used the antibody to stain for MHC II+ cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies MHC II+ cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies MHC II+ cells very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identifying MHC II+ immune cell populations.

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