Easy-to-Use TGF-beta1 ELISA Kit and Less Deviation of Results from both Mouse and Human Cell Line Preparations

May 06, 2020

Institute of Virology, TU Munich
Medicine
Graduate Student

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Company:

invitrogen

Product Name:

Human/Mouse TGF-beta1 Uncoated ELISA kit

Catalog Number:

88-8350-88

I use this kit to determine knockdown of TGFb-1 from cell supernatent of my in-vitro experiments. I have tested this kit for both mouse and human cell lines and it works perfectly.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell Supernatent

Tips

Overnight coating and overnight binding of Antigen is recommended

Results Summary

Cell supernatent from both human (HepG2) and Mouse (AML-12 and RAW264.7) cell lines were tested for TGFb-1 expression. The aim of the experiment was to determine the baseline expression of TGFb-1 secreted by the cells. TGFb-1 knockdown was assessed 96 hours post transfection with ELISA. Also, to reduce the background from FCS, the cells were adapted to serum-free conditions and appropriate media-only controls were run during the experiment.

DOI or PMID #

N/A

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Summary

The Good

Reliable, inexpensive, not expensive, sensitivity

The Bad

Time consumption

The Bottom Line

The kit is quite straight forward to use, the protocol provided is not at all complicated. Although, the ELISA takes about 3 days to finish as two overnight steps are involved. The kit is quite sensitive, I could measure as low as 10pg/ml concentrations of TGFb-1 from the cell supernatent which is quite useful. For the maximum sensitivity it is recommended to let the antigen bind overnight at 4 degree C than at room temperature for 2 hours. I have tried both and overnight works the best. Also this kit is quite inexpensive as compared to other coated ELISA kits. I did get quite reproducible results and hence the kit is very reliable for a series of experiment.

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