Good Detection of Marginal Zone B Cells

April 27, 2021

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD21/CD35 (CR2/CR1) Antibody

Catalog Number:

123407

Clone Number:

7E9

We are interested in detection of marginal zone and follicular zone B cells in mouse spleens

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse splenocytes

Primary Incubation

1:200, 30 min, room temp

Blocking Agent

FC block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZE5 Yeti flow cytometer

Results Summary

We were able to detect marginal zone and follicular zone B cells by staining for CD21 and CD23

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use wit CD23, reliable, bright, distinctive population

The Bad

N/A

The Bottom Line

Easy to use and fit into a panel with CD23, distinctive population

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