CD44 Antibody Works Great

April 22, 2021

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cy7 anti-mouse/human CD44 Antibody

Catalog Number:

103029

Clone Number:

IM7

We are interested in detection of CD44high CD4 T cells in mouse tissues

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, 30 min, room temp

Blocking Agent

FC block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZE5 Yeti flow cytometer

Results Summary

CD44high CD4 T cells were detectable in the spleens of mice immunized with OVA/alum.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

easy to use, bright, distinguishable population

The Bad

N/A

The Bottom Line

Reliable and repeatable

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