Detection of IgG1-Switched B cells

April 16, 2021

microbiology
NYU
Postdoctoral Researcher

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Company:

Biolegend

Product Name:

FITC anti-mouse IgG1 Antibody

Catalog Number:

406605

Clone Number:

RMG1-1

Mice were immunized with OVA Alum and we were interested in the frequency of B cells that had undergone isotype switching between IgM and IgG1

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, room temp, 20 min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

ZE5 flow cytometer

Results Summary

B cells class switched from IgM to IgG1 were detectable by flow cytometry

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, specific

The Bad

Not as bright

The Bottom Line

Repeatable and reliable

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