Detection of Ly6C+ Cells in Mice

May 15, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-mouse Ly-6C Antibody

Catalog Number:

128033

Clone Number:

HK1.4

We are interested in detection of ly6c+ cells in various mouse tissues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, 20 min, room temp

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 YETI flow cytometer

Results Summary

LY6C+ Cells were detectable in the spleens of mice. Same antibody used to detect LY6C+ cells in mouse skin

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright antibody, works very well

The Bad

510 can be hard to use with other BV antibodies

The Bottom Line

Repeatable and reliable

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