This P62 Antibody Is a Good Marker for Autophagy Induction

Biology
Seoul National University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

P62/SQSTM1 Antibody

Catalog Number:

18420-1-AP

Clone Number:

Polyclonal

I use p62 as a control to check the induction of autophagy. This antibody is very good for that purpose.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293T, HeLa, HEK293, HCT116, and COS7

Primary Incubation

1:2000 in 5% non-fat milk

Blocking Agent

5% non-fat milk in TBST buffer

Secondary Incubation

Anti-Rabbit IR-Dye800

Tertiary Incubation

N/A

Detection

Odyssey for quantitative WB

Results Summary

When cell lysates without ATG7 are blotted for p62, I always see the nice increase of p62 level. This is a good control for autophagy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Works for most cell lines

The Bad

Nothing

The Bottom Line

A good antibody for autophagy study.

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