Go-to CD279 Antibody in My Activation/Exhaustion Panel

April 30, 2020

Surgery
NYU Langone Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD279 (PD-1) Antibody

Catalog Number:

135206

Clone Number:

29F.1A12

Fluorophore with brightness index 5 which makes it perfect for even lower levels of PD-1 expression.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine pancreas with orthotopically injected murine pancreas cancer cells

Primary Incubation

Incubated at 4C for 1h, dilution 1:500

Blocking Agent

1% FACS, anti-CD16/CD32, Mouse serum, Rat IgG

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT

Results Summary

Yields continuous right shift with increasing expression levels. Easy to distinguish with and without an FMO.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High brightness index, great staining

The Bad

Relatively expensive

The Bottom Line

Straightforward use with clear staining.

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