Great Anti-Rat CD4 Antibody from BioLegend

April 16, 2020

Medicine
Queen's University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-rat CD4 Antibody

Catalog Number:

201515

Clone Number:

W3/25

We use this antibody to enumerate lymphocytes in rat blood and spleen. This antibody yields a bright signal with very little background staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat blood or splenocytes

Primary Incubation

1:400, 30 min at 4C

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

The antibody gives a bright and clear signal. It does a good job of detecting CD4 T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining.

The Bad

Some slight background staining.

The Bottom Line

A good antibody to detect CD4 T cells in rat samples.

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