Detection of Mouse B Cells in PE/CY7

April 24, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103222

Clone Number:

RA3-6B2

We are interested in detecting mouse B cells in tissues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spenocytes

Primary Incubation

1:200, room temperature, 30 minutes

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 yeti flow cytometer

Results Summary

CD4 T cells and B cells were detected from mouse splenocytes

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Can be used in conjunction with CD4 FITC, easy detection of B cells

The Bad

-

The Bottom Line

Easy and reliable detection

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