Detection of GATA3 High T Cells

April 07, 2020

microbiology
NYU
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PerCP/Cyanine5.5 anti-GATA3 Antibody

Catalog Number:

653812

Clone Number:

16E10A23

We are interested in identifying GATA3high CD4 T cells from the lymph node and lung of allergen-sensitized mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells

Primary Incubation

3uL/test, 30 minutes, room temp

Blocking Agent

FC block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 YETI Biorad flow cytometer

Results Summary

Animals were sensitized with allergen, GATA3-high T cells were identified in the lymph node of allergen-sensitized mice.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Discernable detection of GATA3-low and GATA3-high cells

The Bad

Requires 3uL/test

The Bottom Line

Reliable detection of GATA3.

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